This workshop is hands-on. Attendees will learn how to identify cell populations and detect differentially expressed genes in a simple Single Cell RNA-Seq experiment with the point-and-click interface in Partek Flow.
In this workshop you will learn how to analyze single cell RNA-seq data using Partek software
Location: Farrell Learning and Teaching Center - FLTC Room 304
Date: Thursday, May 23, 2019
Time: 9:30 – 12
Agenda:
Import data, filter cells using interactive QA/QC charts, filter and normalize Single Cell RNA-Seq data
Visualize cell populations using the interactive 3D t-SNE plot, overlay gene expression and pathway signatures on the 3D t-SNE plot
Select and classify cells on the 3D t-SNE plot, detect differentially expressed genes
Filter a gene list, identify enriched KEGG pathway and/or GO terms
Visualize cell-level results using heat maps, volcano plots, and violin plots