Single Cell RNA-Seq Data Analysis in Partek Flow

This workshop is hands-on. Attendees will learn how to identify cell populations and detect differentially expressed genes in a simple Single Cell RNA-Seq experiment with the point-and-click interface in Partek Flow.

In this workshop you will learn how to analyze single cell RNA-seq data using Partek software

Location: Farrell Learning and Teaching Center - FLTC Room 304

Date: Thursday, May 23, 2019

Time: 9:30 – 12

 

Agenda:

  • Import data, filter cells using interactive QA/QC charts, filter and normalize Single Cell RNA-Seq data
  • Visualize cell populations using the interactive 3D t-SNE plot, overlay gene expression and pathway signatures on the 3D t-SNE plot
  • Select and classify cells on the 3D t-SNE plot, detect differentially expressed genes
  • Filter a gene list, identify enriched KEGG pathway and/or GO terms
  • Visualize cell-level results using heat maps, volcano plots, and violin plots

May 23rd, 2019 9:30 AM - 12:00 PM
Farrell Learning and Teaching Center - FLTC 304
United States
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