Single Cell RNA-Seq Data Analysis in Partek Flow

In this workshop you will learn how to analyze single cell RNA-seq data using Partek software

Location: Farrell Learning and Teaching Center - FLTC Room 304

Date: Thursday, May 23, 2019

Time: 9:30 – 12

Agenda:

• Import data, filter cells using interactive QA/QC charts, filter and normalize Single Cell RNA-Seq data
• Visualize cell populations using the interactive 3D t-SNE plot, overlay gene expression and pathway signatures on the 3D t-SNE plot
• Select and classify cells on the 3D t-SNE plot, detect differentially expressed genes
• Filter a gene list, identify enriched KEGG pathway and/or GO terms
• Visualize cell-level results using heat maps, volcano plots, and violin plots